ELISABETH PHARMACON

Description of product lines

Product lines of EliGene

EliGene® kits are divided according to detection chemistry to basic lines RT, LC and UNI, respectively.

In RT line, the TaqMan chemistry using hydrolyzing fluorescence probes is used in the combination with hot-start polymerase and ROX dye as a passive reference. The total reaction volume is 25ul when 5ul of isolated DNA is added directly to ready to use mastermix containing all necessary components for reaction. In RT kits, the fluorescence dyes with excitation wavelengths of 495, 520 nm and emission wavelengths of 520, 550 nm together with non-fluorescence quenchers are used. These kits are basically designed for ABI instruments of 7000 line and they are optimized on LightCycler480 instrument but can be used with other instruments that meet the spectral characteristics of used fluorescence dyes. The typical time of the run for RT kits is about 2 hours.
 
In LC line, the Molecular Beacons chemistry using hybridization fluorescence probes is used in combination with hot-start polymerase. The total reaction volume is 20 ul when 5 ul of isolated DNA is added to mastermix created by mixing of polymerase with reaction mix containing all necessary components for the reaction. The fluorescence dyes with excitation wavelengths of 495, 460 and emission wavelengths of 515, 705 together with non-fluorescence quenchers are used. The LC kits are basically designed for LightCycler 1.2 and 2.0 instruments but they can be used with other instruments, that meets on the one hand the spectral characteristics of used fluorescence dyes in individual versions and on the other hand rapid heating/cooling rate. The typical time of run for LC kits is about 1 hours.

In UNI line, the Molecular Beacons chemistry using hybridization fluorescence probes is used in combination with hot-start polymerase. The total reaction volume is 20 ul when 5 ul of isolated DNA is added directly to ready-to use mastermix containing all necessary components for reaction. The usage of uracil in the reaction mix enables UNG treatment to prevent the re-amplification of carryover-PCR products by removing any uracil incorporated into single- or double-stranded DNA. In UNI kits the fluorescence dyes with excitation wavelengths of 495, 520 and emission wavelengths of 520, 550 together with non-fluorescence quenchers are used. These kits are designed for almost all real-time instruments on the market. The typical time of run for UNI kits is about 50 minutes.

DNA/RNA Isolation

The amplification success depends mostly on the quality of the purified NA. The highest sensitivity in PCR detection can only be achieved when you have first-rate sample preparation. The quality of sample preparation depends not only on isolation procedure but also on timing of collection (clinical relevancy), transport and storage conditions. The presence of conservation agents in transport media could lead to PCR inhibition as well. Taking together all these facts, the isolation procedure must be rigorously tested to avoid false negative results.
EliGene® and MAGNETO isolation kits offer to customer optimized solution with the high quality reagents for manual and automatic preparation. EliGene® and MAGNETO isolation kits are rigorously tested in order to exclude the presence of contaminants and PCR inhibitors. Together with EliGene® detection kits including Internal Control, EliGene® or MAGNETO isolation kits, we provide optimal solution for accurate and high sensitive diagnostics.

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