EliZyme PCR accessories

EliZyme™ Reverse Transcriptase

EliZyme™ Reverse Transcriptase is a thermostable and extremely active modified M-MLV reverse transcriptase and is a reliable and heat-resistant enzyme designed to improve the speed and efficiency of cDNA synthesis while ensuring accurate transcript representation.

Some of the key features of EliZyme™ Reverse Transcriptase include its thermostable nature, which allows for transcription at temperatures between 45 °C and 55 °C, as well as the presence of an advanced RNase inhibitor that prevents RNA degradation by contaminating RNase. Additionally, this product can accurately transcribe GC-rich RNA regions with high secondary structure. The enzyme is provided with a 5X buffer that contains Mg, dNTPs, stabilizers, and enhancers, which have been optimized to generate high cDNA yield from as little as 4pg of RNA.

EliZyme™ Reverse Transcriptase can be used with random hexamer, oligo-dT, and gene-specific primers for cDNA synthesis, which is useful for a variety of downstream applications, such as PCR analysis, cloning, library preparation, and Next Generation Sequencing. It is efficient in synthesizing cDNA from low-copy number transcripts, viral RNA targets, and miRNA targets, and can be used with total RNA or poly(A)+ RNA. EliZyme™ Reverse Transcriptase does not include oligos, providing users with the flexibility to define their own priming strategy based on their specific analytical needs.

Features:

  • Thermostable reverse transcriptase 45 °C to 55 °C
  • Advanced RNase inhibitor
  • High cDNA yields from as little as 4pg total RNA
  • Accurate reverse transcription of GC-rich templates
  • Sensitive detection of low copy number transcripts
  • Advanced buffer chemistry including Mg and dNTPs

Applications:

  • Random hexamer, oligo-dT and gene-specific primers
  • cDNA synthesis for PCR analysis, cloning, library preparation and Next Generation Sequencing
  • Low copy number transcripts
  • Viral RNA targets, miRNA targets
  • Efficient synthesis from total RNA or poly(A)+ RNA

Items Ref. No. Size Package Price ex. VAT VAT  
EliZyme™ Reverse Transcriptase EZ8005-EN* 1x50µl (200U/µl) + 1x200µl buffer 50 rxns € 200.50 21 % Buy

* Transport on dry ice. An additional charge will apply.

EliZyme™ Reverse Transcriptase EZ8020-EN* 4x50µl (200 U/µl) + 4x200µl buffer 200 rxns € 732.00 21 % Buy

* Transport on dry ice. An additional charge will apply.

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