EliZyme for End-point PCR

EliZyme™ HS FAST

EliZyme™ HS FAST is a powerful DNA polymerase that uses advanced hot start technology for improved sensitivity and specificity in PCR applications. The hot-start feature prevents non-specific amplification and primer-dimer formation by inactivating the enzyme below 65 °C, allowing for specific amplification of low copy number target sequences. The antibody-mediated hot start technology provides superior sensitivity and specificity compared to other methods.

EliZyme™ HS FAST is suitable for a wide range of applications, including genotyping, TA cloning, colony PCR, high-throughput workflows, low abundance target detection, routine and multiplex PCR, direct PCR on blood and urine, methylated DNA amplification for bi-sulphite sequencing, and "difficult" PCR on GC/AT-rich templates. The enzyme system delivers increased PCR success rates with amplicons up to 6kb and ultra-low background DNA.

EliZyme™ HS FAST uses advanced buffer chemistry, including Mg and dNTPs, to enhance PCR speed, yield, and specificity. The enzyme is efficient and specific in amplifying GC and AT-rich templates, and has increased inhibitor tolerance. The polymerase is produced using an enhanced 12-step purification strategy that removes host DNA physically, chemically, and enzymatically.

For added convenience, EliZyme™ HS FAST is available as a 2X ready mix that can be easily set up, and EliZyme™ HS FAST MIX Red contains a red dye suitable for direct loading and tracking during agarose gel electrophoresis. All versions of the enzyme perform well across the recommended applications.

Features:

  • Hot start technology for unrivalled detection of low abundance templates
  • Increased PCR success rates with amplicons up to 6kb
  • Ultra-low background DNA
  • Advanced buffer chemistry including Mg and dNTPs
  • High yields with standard or fast cycling
  • Efficient and specific amplification of GC and AT-rich templates
  • Increased inhibitor tolerance

Applications:

  • Genotyping
  • TA cloning
  • Colony PCR
  • High throughput workflows
  • Low abundance target detection
  • Routine and multiplex PCR
  • Direct PCR on blood and urine
  • Methylated DNA amplification for bi-sulphite sequencing
  • Difficult PCR on GC/AT-rich templates

Product variants:

  • EliZyme™ HS FAST MIX
  • EliZyme™ HS FAST MIX Red

Items Ref. No. Size Package Price ex. VAT VAT  
EliZyme™ HS FAST EZ5505-EN 1x0,1ml 5U/µl + 4x1ml buffer 500 U € 295.60 21 % Buy
EliZyme™ HS FAST EZ5510-EN 2x0,1ml 5U/µl + 1x8ml buffer 1000 U € 591.30 21 % Buy
EliZyme™ HS FAST EZ5520-EN 4x0,1ml 5U/µl + 2x8ml buffer 2000 U € 1 182.60 21 % Buy
EliZyme™ HS FAST MIX EZ5720-EN 5x1ml mix 200 rxns € 159.00 21 % Buy
EliZyme™ HS FAST MIX EZ5760-EN 2x7,5ml mix 600 rxns € 443.60 21 % Buy
EliZyme™ HS FAST MIX Red EZ5620-EN 5x1ml mix 200 rxns € 159.00 21 % Buy
EliZyme™ HS FAST MIX Red EZ5660-EN 2x7,5ml mix 600 rxns € 443.60 21 % Buy
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