Sexually Transmitted Urinary Infection

EliGene® Chlamydia Trachomatis
  • EliGene® Chlamydia Trachomatis
  • EliGene® Chlamydia Trachomatis

EliGene® Chlamydia Trachomatis UNI (RUO)

Intended use

EliGene® Chlamydia trachomatis UNI kit is intended for DNA detection of pathogenic bacteria Chlamydia trachomatis from clinical specimens. The kit is based on detection of cryptic plasmid together with genomic DNA of Chlamydia trachomatis bacteria. The kit gives positive result for bacteria Chlamydia trachomatis when also “Sweden variant“ carrying 377 bp deletion in cryptic plasmid and variant lacking cryptic plasmid are detected.

Principle of the method

This detection kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit primers and Molecular Beacons labeled probes (PULSAR and FAM) for the DNA detection of Chlamydia trachomatis and internal control are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

  • Roche: LightCycler® 480, LightCycler® Nano, LightCycler® 2.0
  • Thermofisher Scientific: Quant Studio 3 a 5
  • Applied Biosystems: 7500FAST
  • Qiagen: RotorGene 6000 nebo Q
  • Bio -Rad: CFX96 Touch Real -Time PCR Detection System
  • Bio Molecular Systems: MIC qPCR Cycler

Introduction

Chlamydia is gram-negative bacteria that parasitize inside the susceptible cells due to their disability to synthesise ATP. Genus Chlamydia covers four known species: C. trachomatis, C. psittaci, C. pecorum and C. pneumoniae when C. psittaci and C. pecorum primary belongs to animal parasites. Chlamydia trachomatis is the most frequently reported sexually transmitted pathogen when up to 10 % of population is infected. Chlamydia trachomatis is responsible for several clinically different diseases as cervicitis, pelvic inflammatory disease (PID), infant pneumonia and conjunctivitis (pink eye) in newborns, urethritis, prostatitis or epididymitis. In men Chlamydia trachomatis often cause non-gonococcal urethritis. In women, untreated infection can cause serious problems mainly due to the fact that app. 75 % of these infections are asymptomatic. For this reason there is a lot of non-diagnosed and untreated cases resulted in other problems mainly in pregnant women. Newborns of mothers with cervical Chlamydia infections have very high risk (60–70 %) to be infected. Also these newborns have a very high risk of C. pneumoniae and conjunctivitis (pink eye).

Primary sample collection, handling and storage

Clinical material and Recommended DNA isolation procedure:
Urine, Swabs, Sperm

Manual: EliGene® Urine Isolation Kit
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

Research Use Only (RUO)

Items Ref. No. Package Min. package Price ex. VAT VAT  
EliGene® Chlamydia Trachomatis UNI 90046-UNI 50 reactions 1 kit On request 21 %
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