Human Genetics

EliGene® Lactose intolerance RT

Intended use

EliGene® Lactose intolerance RT kit is intended for the DNA detection of C-13910T and G-22018A polymorphisms located in the regulatory region of the lactase gene from isolated DNA.

Principle of the method

This diagnostic kit is based on RealTime PCR genotyping (allelic discrimination). In this kit primers and dual labeled probes (C-13910T FAM and HEX, G-22018A TexasRed and Cy5) for the detection of single nucleotide polymorphisms C-13910T and G-22018A in MCM6 gene from isolated DNA are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

  • Thermofisher Scientific: Quant Studio 5
  • Bio-Rad: CFX96 Touch Real-Time PCR Detection System

Introduction

In clinical laboratories, patients with lactose intolerance are routinely tested for the presence of single nucleotide polymorphisms C-13910T and G-22018A found in the MCM6 gene, which were associated with genetically determined lactose intolerance encompassing decreased lactase production. Genotyping of these polymorphisms allows to differentiate between lactose intolerance caused by genetic predispositions and that caused by secondary effects. Genotyping test facilitates fast and routine analysis of the above-mentioned polymorphisms in a large number of samples. EliGene® Lactose intolerance RT kit provides right results also within patients carrying the C-13907G, T-13913C, G-13914A and T-13915G mutations.

Clinical material and recommended DNA isolation procedure

Blood, swabs

Manual: EliGene Urine Isolation Kit (ELISABETH PHARMACON)

Automatic: ZEPHYRUS Magneto (ELISABETH PHARMACON)

CE IVD

Items Ref. No. Package Price ex. VAT VAT  
EliGene® Lactose intolerance RT 90092-RT 50 reactions On request 21 %

No questions.

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