Sexually Transmitted Urinary Infection

EliGene® Neisseria
  • EliGene® Neisseria
  • EliGene® Neisseria

EliGene® Neisseria UNI

Intended use

EliGene® Neisseria UNI kit is intended for DNA diagnostic of pathogenic species Neisseria gonorrhoeae from isolated DNA sample.

Principle of the method

This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit primers and Molecular Beacons labeled probes (FAM and HEX) for the detection of Neisseria gonorrhoeae DNA and internal control are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

  • Roche: LightCycler® 480, LightCycler® Nano, LightCycler® 2.0
  • Qiagen: RotorGene 6000 nebo Q
  • Thermofisher Scientific: Quant Studio 3 a 5
  • Applied Biosystems: ABI 7500FAST
  • Bio-Rad: CFX96 Touch Real-Time PCR Detection System
  • Bio Molecular Systems: MIC qPCR Cycler

Introduction

Neisseria gonorrhoeae (gonococci) is gram-negative, non-motile and non-sporulated diplococcic. Of the eleven species of Neisseria colonizing humans, only two are pathogens, N. gonorrhoeae and N. meningitidis. N. gonorrhoeae is the causative agent of sexual transmitted disease gonorrhea which is the third most common sexual disease, worldwide is registered about 62.2 million cases of gonorrhea. In males and females the gonorrhea is clinically manifested as urethritis with a purulent (or pus-like) discharge from the genitals in females also as cervicitis. Complication can be infection in pelvic region leading up to infertility in females. The classical penicillin family of antibiotics is relatively well effective in therapy but the number of resistance strains is increasing. Also the simultaneous infection by Chlamydia infection is common. For diagnostic purposes from clinical specimen the urea and cervical swabs are recommended.

Primary sample collection, handling and storage

Clinical material and recommended DNA isolation procedure:
Urine, swabs

Manual: EliGene® Urine Isolation Kit
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

CE IVD

Items Ref. No. Package Min. package Price ex. VAT VAT  
EliGene® Neisseria UNI 90047-UNI 50 reactions 1 kit On request 21 %
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