Sexually Transmitted Urinary Infection

EliGene® Ureaplasma
  • EliGene® Ureaplasma
  • EliGene® Ureaplasma

EliGene® Ureaplasma UNI

Intended use

EliGene® Ureaplasma UNI kit is intended for DNA diagnostics of pathogenic species Ureaplasma urealyticum and Ureaplasma parvum from isolated DNA sample.

Principle of the method

This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit primers and Molecular Beacons labeled probes (FAM and HEX) for the detection of Ureaplasma urealyticum and Ureaplasma parvum DNA and internal control are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

  • Roche: LightCycler® 480, LightCycler® Nano, LightCycler® 2.0
  • Qiagen: RotorGene 6000 nebo Q
  • Thermofisher Scientific: Quant Studio 3 a 5
  • Applied Biosystems: ABI 7500FAST
  • Bio-Rad: CFX96 Touch Real-Time PCR Detection System
  • Bio Molecular Systems: MIC qPCR Cycler

Introduction

Ureaplasma species colonize the lower urogenital tract in many healthy persons, yet, they can also cause urethritis, endometritis, chorioamnionitis, spontaneous abortion, arthritis and urinary calculi in susceptible adults, as well as prematurity/low birth weight, bacteraemia and meningitis.
Ureaplasma urealyticum is bacterium belonging to the family Mycoplasmataceae. U. urealyticum consists of 14 serovars that can be divided into two biovars, biovars 1 and biovars 2, on the basis of genotypic characteristics. Biovar 1 is made up of four serovars (serovars 1, 3, 6 and 14), whereas biovar 2 comprises ten serovars (serovars 2, 4, 5 and 7–13). Evidence has been presented that the species currently known as U. urealyticum should be separated into two species, namely, U. parvum (previously, U. urealyticum biovar 1) and U. urealyticum (previously, U. urealyticum biovar 2). The majority of human Ureaplasma isolates belong to the new species U. parvum. U. urealyticum (biovar 2) is isolated less often, but is not uncommon.
Ureaplasma sp. is the main cause of non-gonococcal, non-chlamydial urethritis, acute prostatitis and acquired arthritis in men. In pregnant and non-pregnant women, Ureaplasma can cause chorioamnionitis and pre-term delivery, abortion, pre-term birth, vaginitis and cervicitis. Clinical studies have demonstrated that infants born to infected mothers become infected with these bacteria, and colonization of the respiratory tract of infants has been associated with pneumonia, respiratory distress and meningitis.

Primary sample collection, handling and storage

Clinical material and Recommended DNA isolation procedure:
Urine, swabs

Manual: EliGene® Urine Isolation Kit
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

CE IVD

Items Ref. No. Package Min. package Price ex. VAT VAT  
EliGene® Ureaplasma UNI 90049-UNI 50 reactions 1 kit On request 21 %
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