EliZyme for End-Point PCR

EliZyme™ HS HIFI MIX

Advantages

  • 3’-5’ exonuclease (proofreading) activity
  • Adjust the volume of enzyme

Applications

  • Preparation of NGS library
  • Routinne PCR
  • Applications requiring high precision
  • GC-rich and AT-rich sequences
  • Other genotyping applications

EliZyme HS HIFI MIX is characterized by its 3’-5’ exonuclease (proofreading) activity in PCR. Significantly improved performance is attributed to several point-mutations which improve the performance compared with its native form. The error rate of EliZyme HS HIFI MIX is 100x lower than Taq DNA polymerase.

Improved processivity, higher yield and shortening cycling times are ensured by enhanced DNA binding.  PCR inhibition from impure samples such as colony PCR and direct PCR is minimised by enhanced efficiency of EliZyme HS HIFI MIX polymerase.

EliZyme HS HIFI MIX is characterized by enhanced PCR speed, yield and specificity. The combination of enzyme and buffer system makes exceptional PCR performance on complex templates such as mammalian genomic DNA.

Ref. No. Size Package Price ex. VAT VAT  
EZ2501 1x1.25ml mix 100 rxns € 136.90 21 % Buy
EZ2505 1x6,25ml mix 500 rxn € 649.30 21 % Buy
EZ2510 2x6.25ml mix 1000 rxns € 1 253.30 21 % Buy

MSDS EliZyme HS HIFI MIX CZ

(published 16.03.2018, size 559.4 kB)

Návod k použití EliZyme HS HIFI MIX

(published 24.08.2023, size 898.3 kB)

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