Viral Infections

EliGene® Enterovirus
  • EliGene® Enterovirus
  • EliGene® Enterovirus

EliGene® Enterovirus LC

Intended use

EliGene® Enterovirus LC is intended for the detection and the quantification of Enterovirus RNA.

Principle of the method

This diagnostic kit is based on Reverse Transcription quantitative PCR method in one tube. In this kit primers and labeled probes (FAM and HEX) for the detection of Enterovirus RNA and for the detection of internal control are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

  • Roche: LightCycler® 480
  • Thermofisher Scientific: Quant Studio 3 a 5
  • Qiagen: RotorGene Q
  • Bio-Rad: CFX96 Touch Real-Time PCR Detection System

Introduction

Human Enterovirus is a genus of (+)ssRNA viruses associated with several human and mammalian diseases.
Until now, 66 serotypes are known of which there are 62 non-polio Enteroviruses that can cause disease in humans: 23 Coxsackie A viruses, 6 Coxsackie B viruses, 28 echoviruses, and 5 other Enteroviruses. Enteroviruses are ubiquitous pathogens with a high incidence worldwide (ca. 500 million infections/year) and are often found in the respiratory secretions (e.g., saliva, sputum, or nasal mucus) and stool of an infected person. Enteroviruses may cause life-threatening infections, especially among children when diseases such as myocarditis, paralysis, multiple organ failure, meningitis and encephalitis may be associated with infections.

Primary sample collection, handling and storage

Clinical material and recommended DNA isolation procedure:

CSF, serum, plasma

Manual: Chemagic Viral DNA/RNA Kit (Chemagen-PerkinElmer)
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

CE IVD

Items Ref. No. Package Min. package Price ex. VAT VAT  
EliGene® Enterovirus LC 90053-LC 50 reactions 1 kit On request 21 %

Instructions for use EliGene Enterovirus LC

(published 01.04.2020, size 412.3 kB)

MSDS EliGene Enterovirus Enzyme Mix EN

(published 12.05.2023, size 224.1 kB)

MSDS EliGene Enterovirus IC RNA EN

(published 12.05.2023, size 223.7 kB)

MSDS EliGene Enterovirus Mix EN

(published 12.05.2023, size 209.9 kB)

MSDS EliGene Enterovirus PC DNA EN

(published 12.05.2023, size 210.2 kB)

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