EliGene® Norovirus LC

Intended use

EliGene® Norovirus LC is intended for the detection and the quantification of Norovirus GI and GII RNA.

Principle of the method

This diagnostic kit is based on Reverse Transcription-quantitative PCR method in one tube. In this kit primers and labeled probes (FAM and HEX) for the detection of Norovirus RNA and for the detection of internal control are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

• Roche: LightCycler® 480
• Thermofisher Scientific: Quant Studio 3 a 5
• Qiagen: RotorGene Q
• Bio-Rad: CFX96 Touch Real-Time PCR Detection System

Introduction

Noroviruses are a genetically diverse group of single-stranded RNA, non enveloped viruses in the Caliciviridae family. The viruses are transmitted by fecally contaminated food or water, by person-to-person contact, and via aerosolization of the virus and subsequent contamination of surfaces. Noroviruses are the most common cause of viral gastroenteritis in humans. Norovirus affects people of all ages. Outbreaks of norovirus infection often occur in closed or semiclosed communities, such as long-term care facilities, overnight camps, hospitals, prisons, dormitories, and cruise ships, where the infection spreads very rapidly either by person-to-person transmission or through contaminated food. Norovirus is rapidly inactivated by either sufficient heating or by chlorine-based disinfectants, but the virus is less susceptible to alcohols and detergents, as it does not have a lipid envelope.

Primary sample collection, handling and storage

feces, water

Manual: PowerMicrobiome™ RNA Isolation Kit, PowerWater® RNA Isolation Kit

CE IVD