Respiratory infections

EliGene® Legionella pneumophila
  • EliGene® Legionella pneumophila
  • EliGene® Legionella pneumophila

EliGene® Legionella pneumophila UNI

Intended use

EliGene® Legionella pneumophila UNI is intended for DNA diagnostics of pathogenic species Legionella pneumophila from isolated DNA sample.

Principle of the method

This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit for the detection of Legionella pneumophila and internal control primers and Molecular Beacons labeled probes (FAM and HEX) are used.

Introduction

Legionella pneumophila is a thin, aerobic, gram-negative, non-encapsulated, aerobic bacillus of the genus Legionella containing more than 50 species. It is the best-characterized member of the genus and major causative agent of Legionnaires' disease, a severe form of acute pneumonia. L. pneumophila is an intracellular pathogen when the bacteria invades and replicates in alveolar macrophages and epithelial cells. Macrolides (azithromycin) or fluoroquinolones (moxifloxacin) are the standard treatment for L. pneumophila in humans. About 3 to 8% of all community-acquired pneumonias are caused by L. pneumophilla when the case-fatality rate for patients with legionellosis is between to 30% with elderly and immunocompromised patients at greater risk of death.

Primary sample collection, handling and storage

Clinical material and recommended DNA isolation procedure:
Throat swabs, Nasopharyngeal aspirates, Sputum     

Manual: EliGene® Urine Isolation Kit
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

CE IVD

Items Ref. No. Package Min. package Price ex. VAT VAT  
UNI EG-90056-UNI 50 reactions 1 kit On request 21 %

Instructions for use EliGene Legionella pneumophila UNI

(published 12.12.2018, size 787.1 kB)

MSDS Eli Blocker EN

(published 25.07.2018, size 541.0 kB)

MSDS EliGene Legionella pneumophila UNI L. pneumophila UNI Mix EN

(published 25.07.2018, size 528.5 kB)

MSDS EliGene Legionella pneumophila UNI PC DNA L. pneumophila EN

(published 25.07.2018, size 528.5 kB)

MSDS IC DNA 01 EN

(published 25.07.2018, size 547.3 kB)

MSDS ROX dye EN

(published 25.07.2018, size 540.6 kB)

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