Respiratory infections

EliGene® Mycoplasma pneumoniae
  • EliGene® Mycoplasma pneumoniae
  • EliGene® Mycoplasma pneumoniae

EliGene® Mycoplasma pneumoniae UNI

Intended use

EliGene® Mycoplasma pneumoniae UNI is intended for DNA diagnostics of pathogenic species Mycoplasma pneumoniae from isolated DNA sample.

Principle of the method

This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit for the detection of Mycoplasma pneumoniae and internal control primers and Molecular Beacons labeled probes (FAM and HEX) are used.

Introduction

Mycoplasma pneumoniae belonging to class Mollicutes has one of the smallest known genomes. It lacks a peptidoglycan cell wall and thus it is resistant to the effects of penicillins and other beta-lactam antibiotics. In humans it causes atypical pneumonia. M. pneumoniae infects the upper and lower respiratory tracts, leading to upper respiratory tract infection, bronchiolitis, tracheobronchitis, bronchitis and community-acquired pneumonia. Infections of the lower respiratory tract generally manifest with a cough, sometimes with dyspnoea, adenopathy, wheezing and, rarely, with respiratory failure. Although M. pneumoniae infections are usually mild, and many are asymptomatic, they are not always self-limiting. M. pneumoniae causes up to 40% of cases of community-acquired pneumonia, but M. pneumoniae respiratory tract infections are also associated with a wide range of extra-pulmonary manifestations, including neurological, cardiac, dermatological, musculoskeletal, haematological and gastrointestinal symptoms.

Primary sample collection, handling and storage

Clinical material and recommended DNA isolation procedure:
Throat swabs, Nasopharyngeal aspirates, Sputum     

Manual: EliGene® Urine Isolation Kit
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

Ref. No. Package Min. package Price ex. VAT VAT  
90055-UNI 50 reactions 1 kit On request 21 %

Instructions for use EliGene Mycoplasma pneumoniae UNI

(published 12.12.2018, size 788.0 kB)

MSDS Eli Blocker EN

(published 25.07.2018, size 541.0 kB)

MSDS IC DNA 01 EN

(published 25.07.2018, size 547.3 kB)

MSDS Mycoplasma pneumoniae UNI M. pneumoniae UNI Mix EN

(published 25.07.2018, size 529.0 kB)

MSDS Mycoplasma pneumoniae UNI PC DNA M. pneumoniae EN

(published 25.07.2018, size 528.6 kB)

MSDS ROX dye EN

(published 25.07.2018, size 540.6 kB)

Related Products