Respiratory infections

EliGene® Influenza A/B/pandemic
  • EliGene® Influenza A/B/pandemic
  • EliGene® Influenza A/B/pandemic

EliGene® Influenza A/B/pandemic

Intended use

EliGene® Influenza A/B/Pandemic LC kit is intended for the detection of Influenza A and B RNA together with Pandemic variant A/H1N1.

Principle of the method

This diagnostic kit is based on Reverse Transcription-quantitative PCR method in one tube. In this kit primers and labeled probes (FAM, HEX and Cy5) for the detection of Influenza A, B, A/H1N1 RNA and for the detection of internal control are used.

Introduction

Influenza is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The most common symptoms are chills, fever, runny nose, sore throat, muscle pains, headache, coughing, weakness and general discomfort. Approximately 33% of people with influenza are asymptomatic. Flu can occasionally lead to pneumonia, either direct viral pneumonia or secondary bacterial pneumonia, even for persons who are usually very healthy. Typically, influenza is transmitted through the air by coughs or sneezes, creating aerosols containing the virus. Influenza can also be transmitted by direct contact with bird droppings or nasal secretions, or through contact with contaminated surfaces. Influenza viruses can be inactivated by sunlight, disinfectants and detergents. Often, new influenza strains appear when an existing flu virus spreads to humans from another animal species, or when an existing human strain picks up new genes from a virus that usually infects birds or pigs. An avian strain named H5N1 raised the concern of a new Pandemic influenza after it emerged in Asia in the 1990s, but it has not evolved to a form that spreads easily between people. In April 2009 a novel flu strain evolved that combined genes from human, pig, and bird flu. Initially dubbed "swine flu" is also known as influenza A/H1N1. Influenza B almost exclusively infects humans and is less common than influenza A. This type of influenza mutates at a rate 2–3 times slower than type A.

Primary sample collection, handling and storage

Clinical material and Recommended DNA isolation procedure:
CSF, serum, plasma, swabs

Manual Isolation:

  • EliGene® Viral RNA/DNA FAST Isolation Kit (Elisabeth Pharmacon) - The quickest (15 minutes) protocol for rapid isolation of clinical samples from swabs
  • Chemagic Viral DNA/RNA Kit (Chemagen-PerkinElmer),
  • QIAamp DSP Virus Spin Kit (Qiagen)

Automatic Isolation:

CE IVD

Items Ref. No. Package Min. package Price ex. VAT VAT  
LC EG-90058-LC 50 reactions 1 kit On request 21 %

Influenza Adv_Notice_042020EN

(published 09.11.2020, size 302.2 kB)

Instructions for use EliGene Influenza A/B/pandemic LC

(published 09.11.2020, size 408.2 kB)

MSDS EliGene Influenza A/B/pandemic LC Enzyme Mix EN

(published 09.11.2020, size 212.1 kB)

MSDS EliGene Influenza A/B/pandemic LC InfA/H1N1 LC Mix EN

(published 25.07.2018, size 528.7 kB)

MSDS EliGene Influenza A/B/pandemic LC InfB LC Mix EN

(published 25.07.2018, size 528.8 kB)

MSDS EliGene Influenza A/B/pandemic PC DNA Influenza EN

(published 09.11.2020, size 216.3 kB)

MSDS IC RNA EN

(published 09.11.2020, size 211.8 kB)

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