Respiratory infections

EliGene® Mycoplasma pneumoniae
  • EliGene® Mycoplasma pneumoniae
  • EliGene® Mycoplasma pneumoniae

EliGene® Mycoplasma pneumoniae UNI

Intended use

EliGene® Mycoplasma pneumoniae UNI is intended for DNA diagnostics of pathogenic species Mycoplasma pneumoniae from isolated DNA sample.

Principle of the method

This diagnostic kit is based on RealTime PCR method using Molecular Beacons hybridization probes. In this kit for the detection of Mycoplasma pneumoniae and internal control primers and Molecular Beacons labeled probes (FAM and HEX) are used.

Compatible instruments

The following list represents instruments on which the above mentioned kits were tested. Please contact us for compatibility with other instruments.

  • Roche: LightCycler 2.0, LightCycler® 480, LightCycler® Nano
  • Thermofisher Scientific: Quant Studio 3 a 5
  • Applied Biosystems: 7500FAST
  • Qiagen: RotorGene 6000 nebo Q
  • Bio-Rad: CFX96 Touch Real-Time PCR Detection System
  • Bio Molecular Systems: MIC qPCR Cycler

Introduction

Mycoplasma pneumoniae belonging to class Mollicutes has one of the smallest known genomes. It lacks a peptidoglycan cell wall and thus it is resistant to the effects of penicillins and other beta-lactam antibiotics. In humans it causes atypical pneumonia. M. pneumoniae infects the upper and lower respiratory tracts, leading to upper respiratory tract infection, bronchiolitis, tracheobronchitis, bronchitis and community-acquired pneumonia. Infections of the lower respiratory tract generally manifest with a cough, sometimes with dyspnoea, adenopathy, wheezing and, rarely, with respiratory failure. Although M. pneumoniae infections are usually mild, and many are asymptomatic, they are not always self-limiting. M. pneumoniae causes up to 40% of cases of community-acquired pneumonia, but M. pneumoniae respiratory tract infections are also associated with a wide range of extra-pulmonary manifestations, including neurological, cardiac, dermatological, musculoskeletal, haematological and gastrointestinal symptoms.

Primary sample collection, handling and storage

Clinical material and recommended DNA isolation procedure:
Throat swabs, Nasopharyngeal aspirates, Sputum     

Manual: EliGene® Urine Isolation Kit
Automatic: MAGNETO BodyFluid DNA/RNA Isolation Kit (for equipment ZEPHYRUS Magneto)

CE IVD

Items Ref. No. Package Min. package Price ex. VAT VAT  
EliGene® Mycoplasma pneumoniae UNI 90055-UNI 50 reactions 1 kit On request 21 %

No questions.

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